Simplified HPLC method for 5-aminosalicylic acid in plasma and urine
Summary
Type: HPLC Application #: 103330
Catalog#: 720023.46 - MN HPLC Column, Nucleosil C18, 100A, 10um, 4.6x250mm
Detection: fluorescence, excitation 300nm, emission 418nm Conditions: eluent: deionized water adjusted to pH 3 with conc. perchloric acid - methanol - acetonitrile (75:12.5:12.5) flow rate 0.5 - 0.6ml/min pressure about 300bar Authors: Fischer C. et al. Source: J. Chromatogr. 225 (1981) 498–503
Details
Legend: Fig. 1. HPLC separation of a) 50ng each of 5AS, 5AcAS and AcPAS, injected
directly into the HPLC system, b) extract of 500µl blank plasma spiked with
internal standard, c) and d) extracts of 500µl b